# # - eval = FALSE --------------------------------------------------------------- # gs_split_by_tree (x) # gs_check_redundant_nodes (x) # gs_remove_redundant_nodes (x, toRemove) # gs_remove_redundant_channels (gs , ...) ## ---- 回声= FALSE、消息= FALSE结果= '隐藏 '-------------------------------- 库(flowWorkspace) flowDataPath < -系统。file("extdata"， package = "flowWorkspaceData") gs <- load_gs(file.path(flowDataPath，"gs_manual")) gs1 <- gs_clone(gs) sampleNames(gs1) <- "1.执行以下命令:fcs" #简单的树节点<- gs_get_pop_paths(gs1) for(toRm in nodes[grepl("CCR"， nodes)]) gs_pop_remove(gs1, toRm) #删除两个终端节点gs2 <- gs_clone(gs1) sampleNames(gs2) <- "2.删除两个终端节点。fcs”gs_pop_remove (gs2 DPT) gs_pop_remove (gs2, DNT) #删除汗衫门gs3 < - gs_clone (gs2) gs_pop_remove (gs3,“汗衫”)gs_pop_add (gs3, gs_pop_get_gate (gs2“CD3 +”),父母=“不碎片”)为(tsub c (CD4, CD8)) {gs_pop_add (gs3, gs_pop_get_gate (gs2 tsub),父母=“CD3 +”)为(toAdd gs_pop_get_children (gs2 tsub)) {thisParent < - gs_pop_get_parent (gs2 [[1]], toAdd,路径=“汽车”)gs_pop_add (gs3, gs_pop_get_gate (gs2 toAdd),父母= thisParent)}} sampleNames (gs3) <——“3。gs4 <- gs_clone(gs3) # rm cd4分支第一gs_pop_remove(gs4，“cd4”)#把它加回来gs_pop_add(gs4，“cd4”)，parent =“CD3+”)#把所有的孩子加回来(toAdd in gs_pop_get_children(gs3，“cd4”)){thisParent <- gs_pop_get_parent(gs3[[1]]， toAdd) gs_pop_get_gate(gs3, toAdd)， parent = thisParent)} sampleNames(gs4) <- "4. "fcs" gs5 <- gs_clone(gs4) #添加另一个冗余节点gs_pop_add(gs5, gs_pop_get_gate(gs， "CD4/CCR7+ 45RA+")[[1]]， parent = "CD4") gs_pop_add(gs5, gs_pop_get_gate(gs， "CD4/CCR7+ 45RA-")[[1]]， parent = "CD4") sampleNames(gs5) <- "5. gs_pop_get_gate(gs， "CD4/CCR7+ 45RA-")Fcs " library(knitr) opts_chunk$set(图。显示=“持有”,fig.width = 4, fig.height = 4,结果= '飞机 ') ## ---- 回声= FALSE --------------------------------------------------------------- 情节(gs1)情节(gs2) # #——回声= FALSE --------------------------------------------------------------- 情节(gs2) (gs3的阴谋 ) ## ----------------------------------------------------------------------------- 看不见(gs_pop_set_visibility (gs2,“汗衫”,假的)阴谋(gs2)情节(gs3) # #——结果= ' '----------------------------------------------------------- gs_get_pop_paths (gs2) [5] gs_get_pop_paths (gs3)[5] # #——结果= ' '----------------------------------------------------------- gs_get_pop_paths (gs2、路径=“汽车”)[5]gs_get_pop_paths (gs3、路径=“汽车”)[5]# #——回声= FALSE --------------------------------------------------------------- # 恢复gs2无形(gs_pop_set_visibility (gs2,“汗衫”,真正的 )) ## ---- 回声= FALSE --------------------------------------------------------------- 情节(gs3)情节(gs4 ) ## ----------------------------------------------------------------------------- gslist < -列表(gs1, gs2 gs3, gs4, gs5) gs_groups < - gs_split_by_tree (gslist)长度(gs_groups) # #——错误= TRUE --------------------------------------------------------------- res < -尝试(gs_check_redundant_nodes (gs_groups),沉默= TRUE)打印(res [[1 ]]) ## ----------------------------------------------------------------------------- 在gs_groups (gp)情节(gp [[1 ]]) ## ----------------------------------------------------------------------------- (我的c(2、4))为(gs gs_groups[[我]])看不见(gs_pop_set_visibility (gs、“汗衫”,假 )) ## ----------------------------------------------------------------------------- 暴雨< - gs_check_redundant_nodes (gs_groups)暴雨# #——消息= FALSE ----------------------------------------------------------- gs_plot_diff_tree (gs_groups) # #——结果= '隐藏 '----------------------------------------------------------- gs_remove_redundant_nodes (gs_groups暴雨 ) ## ----------------------------------------------------------------------------- GatingSetList (gslist ) ## ----------------------------------------------------------------------------- gs_remove_redundant_channels (gs1)