# # - eval = FALSE ---------------------------------------------------------- # groupByTree (x) # checkRedundantNodes (x) # dropRedundantNodes (x, toRemove) # dropRedundantChannels (gs , ...) ## ---- 回声= FALSE、消息= FALSE结果= '隐藏 '--------------------------- 库(flowWorkspace) flowDataPath < -系统。file("extdata"， package = "flowWorkspaceData") gs <- load_gs(file.path(flowDataPath，"gs_manual")) gs1 <- clone(gs) sampleNames(gs1) <- "1. "fcs" #简单树节点<- getNodes(gs1) for(toRm in nodes[grepl("CCR"， nodes)]) Rm(toRm, gs1) #删除两个终端节点gs2 <- clone(gs1) sampleNames(gs2) <- "2.删除两个终端节点。fcs" Rm("DPT"， gs2) Rm("DNT"， gs2) # remove singlets gate gs3 <- clone(gs2) Rm("singlets"， gs3) add(gs3, getGate(gs2， "CD3+")， parent =" not debris") for(tsub in c("CD4"， "CD8")) {add(gs3, getGate(gs2, tsub)， parent =" CD3+") for(toAdd in getChildren(gs2, tsub)) {thisParent <- getParent(gs2[[1]]， toAdd,path="auto") add(gs3, getGate(gs2, toAdd)， parent = thisParent)}} sampleNames(gs3) <- "3. "fcs“#旋转分支使其同质gs4 <-克隆(gs3) # rm cd4分支第一rm(“cd4”，gs4) #将其添加回来add(gs4, getGate(gs3，“cd4”)，parent =“CD3+”)#将所有的孩子添加回来(toAdd in getChildren(gs3，“cd4”)){thisParent <- getParent(gs3[[1]]， toAdd) add(gs4, getGate(gs3, toAdd)， parent = thisParent)} sampleNames(gs4) <- "4. "fcs" gs5 <-克隆(gs4) #添加另一个冗余节点add(gs5, getGate(gs， "CD4/CCR7+ 45RA+")[[1]]， parent = "CD4") add(gs5, getGate(gs， "CD4/CCR7+ 45RA-")[[1]]， parent = "CD4") sampleNames(gs5) <- "5.)Fcs " library(knitr) opts_chunk$set(图。显示=“持有”,fig.width = 4, fig.height = 4,结果= '飞机 ') ## ---- 回声= FALSE ---------------------------------------------------------- 情节(gs1)情节(gs2) # #——回声= FALSE ---------------------------------------------------------- 情节(gs2) (gs3的阴谋 ) ## ------------------------------------------------------------------------ 看不见(setNode (gs2,“汗衫”,假的)阴谋(gs2)情节(gs3) # #——结果= ' '------------------------------------------------------ getNodes (gs2) [5] getNodes (gs3)[5] # #——结果= ' '------------------------------------------------------ getNodes (gs2、路径=“汽车”)[5]getNodes (gs3、路径=“汽车”)[5]# #——回声= FALSE ---------------------------------------------------------- # 恢复gs2无形(setNode (gs2,“汗衫”,真正的 )) ## ---- 回声= FALSE ---------------------------------------------------------- 情节(gs3)情节(gs4 ) ## ------------------------------------------------------------------------ gslist < -列表(gs1, gs2 gs3, gs4, gs5) gs_groups < - groupByTree (gslist)长度(gs_groups) # #——错误= TRUE ---------------------------------------------------------- res < -尝试(checkRedundantNodes (gs_groups),沉默= TRUE)打印(res [[1 ]]) ## ------------------------------------------------------------------------ 在gs_groups (gp)情节(gp [[1 ]]) ## ------------------------------------------------------------------------ (我的c(2、4))为(gs gs_groups[[我]])看不见(setNode (gs、“汗衫”,假 )) ## ------------------------------------------------------------------------ 暴雨< - checkRedundantNodes (gs_groups)暴雨# #——消息= FALSE ------------------------------------------------------ plot_diff_tree (gs_groups) # #——结果= '隐藏 '------------------------------------------------------ dropRedundantNodes (gs_groups暴雨 ) ## ------------------------------------------------------------------------ GatingSetList (gslist ) ## ------------------------------------------------------------------------ dropRedundantChannels (gs1)