## ----库，消息= false -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------库（DESEQ2）库（EDGER）库（变形）## ----计数--------------------------------------------------------------------------------------------------------------------------------------------------------------- counts =simulaternaseqdata（）## ----大号-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------组= rep（c（“ a”，“ b”），每个= 3）dge = dgelist（counts，group = group）dge ## ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ dds = as.deseqdataset（dge）dds ## --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- dge，as。dgelist（DDS））## -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- dds1 = DESeqDataSetFromMatrix(counts, data.frame(condition=group), ~ condition) dds2 = DESeqDataSetFromMatrix(counts, data.frame（条件=组），〜条件）相同（DDS1，DDS2）## ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------- = simulaternaseqdata（output =“ rangedsummarizedExperiment”）SE ## -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- dds = DESeqDataSet(se，设计=〜条件）## ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- dge = as.DGEList(dds) dge ## ----dgeSE--------------------------------------------------------------------------------------------------------名称（Coldata（se））=“ group” dge = dgelist（se）dge ## ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ dds = as.DESeqDataSet(dge) rse = as(dds, "RangedSummarizedExperiment") rse ## ----sessionInfo-------------------------------------------------------------- sessionInfo()