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Views
Votes
Showing :
Normalization
•
reset
1
Vote
3
Replies
57
Views
Normalization for two bulk RNA datasets with different library sizes
Normalization
BulkRNA-seq
DESeq2
1 day ago
K.
• 0
• updated 1 day ago
Michael Love
31k
0
Votes
1
Reply
64
Views
错误使用cqn的包
Normalization
cqn
5 weeks ago
pegah.taklifi
• 10
• updated 5 weeks ago
James W. MacDonald
55k
2
Votes
2
Replies
91
Views
Normalisation in DESeq2
DESeq2
Normalization
7 weeks ago • updated 6 weeks ago
Bine
• 0
1
Vote
2
Replies
136
Views
Analyzing publicly available DESeq2 normalized counts for differential expression
DifferentialExpression
DESeq2
Normalization
8 weeks ago
krovi138
• 0
• updated 7 weeks ago
Michael Love
31k
2
Votes
3
Replies
135
Views
Normalization for within-sample gene correlation
edgeR
Normalization
DESeq2
tpm
RNASeq
8 weeks ago
tcalvo
• 40
• updated 8 weeks ago
Gordon Smyth
41k
0
Votes
2
Replies
89
Views
extract certain subpopulation of MouseGastrulationData
MouseGastrulationData
Normalization
BatchEffect
8 weeks ago
xyang2
• 120
• updated 8 weeks ago
Jonathan Griffiths
• 30
0
Votes
3
Replies
453
Views
Is it possible in DiffBind to specify sizeFactors for DESeq2 analysis?
diffbind
normalization
4.5 years ago
nfursova.msu
• 0
• updated 10 weeks ago
Rory Stark
♦ 3.5k
1
Vote
4
Replies
768
Views
MAnorm output as DiffBind input.
MAnorm
diffbind
normalization
chipseq
3.0 years ago
ajeet
• 0
• updated 10 weeks ago
Rory Stark
♦ 3.5k
0
Votes
1
Reply
173
Views
Normalisation of protein micro array data using VSN
microarray
normalization
protein
19 months ago
keniajin
• 0
• updated 10 weeks ago
Wolfgang Huber
13k
0
Votes
10
Replies
204
Views
Do I have clustering by library size?
PCA
DESeq2
Normalization
librarysize
11 weeks ago
pkachroo
• 10
• updated 10 weeks ago
Michael Love
31k
1
Vote
8
Replies
232
Views
Spike-in normalization in EdgeR
CUTandRUN
edgeR
Normalization
SpikeIn
ChIPSeq
11 weeks ago • updated 10 weeks ago
Hesh
• 0
1
Vote
1
Reply
193
Views
News:
Extensive updates in DiffBind 3.0
Normalization
ATACSeq
ChIPSeq
DiffBind
chipseq
11 weeks ago
Rory Stark
♦ 3.5k
7
Votes
8
Replies
283
Views
How to get gene length for RPKM directly from DGEList object in latest edgeR?
Normalization
edgeR
RPKM
12 weeks ago
anikng
• 0
• updated 12 weeks ago
Gordon Smyth
41k
1
Vote
5
Replies
134
Views
DESeq2 Normalization causing overcorrection in libaries sizes for a few samples
deseq2
normalization
12 weeks ago
asrour
• 0
• updated 12 weeks ago
Michael Love
31k
2
Votes
7
Replies
210
Views
EdgeR diffSpliceDGE: Obtaining exon-level counts and normalisation step
edger
normalization
differential exon usage
splicing
3 months ago
oakhamwolf
• 0
• updated 3 months ago
Yunshun Chen
• 690
1
Vote
5
Replies
111
Views
DESeq2 library composition adjustment in size factors
deseq2
normalization
3 months ago
twfs
• 0
• updated 3 months ago
Michael Love
31k
1
Vote
5
Replies
94
Views
technical replicates and normalization/log transformation
deseq2
normalization
4 months ago
A
• 40
• updated 4 months ago
Michael Love
31k
0
Votes
2
Replies
80
Views
can negative value microarray matrix data analysed by limma directly
limma
microarray
normalization
4 months ago
linouhao
• 0
• updated 4 months ago
Kevin Blighe
2.3k
1
Vote
2
Replies
79
Views
tcga and geo train and validation sets
deseq2
normalization
4 months ago
linouhao
• 0
• updated 4 months ago
Michael Love
31k
0
Votes
2
Replies
77
Views
How to normalise data in ballgown?
r
normalization
edger
4 months ago
lakshmi9c
• 0
• updated 4 months ago
James W. MacDonald
55k
2
Votes
4
Replies
108
Views
Deseq2's geometric mean calculation and 0s
deseq2
normalization
4 months ago
lmrogers34
• 0
• updated 4 months ago
Michael Love
31k
6
Votes
13
Replies
343
Views
How to use RSEM value to find differentially expressed genes
RSEM
limma
edgeR
voom
normalization
4 months ago
harelarik
• 50
• updated 4 months ago
Gordon Smyth
41k
2
Votes
3
Replies
102
Views
Differential gene expression using Ballgown
r
normalization
4 months ago
lakshmi9c
• 0
1
Vote
2
Replies
71
Views
Unexpected rlog values when count spread is large
deseq2
normalization
5 months ago
eric.blanc
• 0
• updated 5 months ago
Michael Love
31k
2
Votes
8
Replies
235
Views
Differential gene expression using R
deseq2
edger
normalization
limma
5 months ago
lakshmi9c
• 0
• updated 5 months ago
Gordon Smyth
41k
2
Votes
4
Replies
477
Views
Deconvolution with CIBERSORT: input data
normalization
r
vst
cibersort
5 months ago
Matan G.
• 30
• updated 5 months ago
Kevin Blighe
2.3k
2
Votes
2
Replies
87
Views
Using EdgeR after importing transcript abundance (by SALMON) with tximport
edger
normalization
5 months ago
ahmedaalkarim
• 0
• updated 5 months ago
James W. MacDonald
55k
0
Votes
1
Reply
58
Views
Input of tscR
microarray
normalization
tscR
time-course
5 months ago
keremwai
• 0
• updated 5 months ago
Kevin Blighe
2.3k
0
Votes
0
Replies
76
Views
EDASeq: is it possible to normalize within lanes for more than one covariate?
EDASeq
normalization
GC
length
RNASeq
5 months ago
abf
• 30
0
Votes
2
Replies
82
Views
ChIP-seq MA plot possibly skewed - how to normalise?
DiffBind
MA plot
normalization
ChIP-seq
5 months ago
hasse.bossenbroek
• 0
• updated 5 months ago
Rory Stark
♦ 3.5k
2
Votes
6
Replies
209
Views
Question: Method to get normalize allele specific read counts
edger
normalization
6 months ago
hemantcnaik
• 0
• updated 6 months ago
Aaron Lun
♦ 26k
0
Votes
0
Replies
38
Views
According to SCBN method, is it possible to acquire normalized data for virtualziation?
SCBN
normalization
6 months ago
tofukaj
• 0
7
Votes
10
Replies
147
Views
is there a function in edger the same as vst or rlog in deseq2
edger
normalization
deseq2
7 months ago
linouhao
• 0
• updated 7 months ago
Gordon Smyth
41k
2
Votes
1
Reply
128
Views
Cross-species analysis and normalization with DESeq2
normalization
deseq2
across species
rnaseq
7 months ago
rmurray2050
• 0
• updated 7 months ago
Michael Love
31k
4
Votes
10
Replies
116
Views
how to analyze WGCNA results?
cancer
probe
deseq2
annotation
normalization
8 months ago
rajpal22288
• 10
• updated 8 months ago
Kevin Blighe
2.3k
0
Votes
1
Reply
98
Views
DESeq2 and Heatmap for multiple conditions
deseq2
normalization
8 months ago
Hamidreza Hashemi
• 20
• updated 8 months ago
Michael Love
31k
2
Votes
7
Replies
126
Views
ControlGenes/ housekeeping genes Deseq2
normalization
deseq2
sizefactors
8 months ago
mankadeep2
• 20
• updated 8 months ago
Michael Love
31k
4
Votes
3
Replies
877
Views
How to plot the batch corrected values in DESeq2
deseq2
normalization
2.0 years ago
dhwani.dholakia
• 0
• updated 8 months ago
Michael Love
31k
0
Votes
4
Replies
394
Views
pheatmap for DEGs
normalization
pheatmap
8 months ago
Hamidreza Hashemi
• 20
• updated 8 months ago
Kevin Blighe
2.3k
0
Votes
2
Replies
133
Views
DESeq2/EdgeR for differential binding analaysis after RepEnrich2
deseq2
edgeR
repenrich2
normalization
9 months ago
mkmwong
• 0
0
Votes
3
Replies
53
Views
Unbalanced design and large difference in number of input reads for DESeq2
deseq2
normalization
9 months ago
jillian.waters
• 0
• updated 9 months ago
Michael Love
31k
0
Votes
2
Replies
53
Views
Error in DESeqDataSet: variables and colnames don't match when they do
deseq2
normalization
9 months ago
ericmalekos
• 0
• updated 9 months ago
Bob Thurman
• 0
0
Votes
3
Replies
58
Views
错误:依赖‘AnnotationDbi’not available for package 'hgu133plus2cdf'
normalization
9 months ago
shenjun_renji
• 0
• updated 9 months ago
Gordon Smyth
41k
0
Votes
1
Reply
53
Views
Removing low sequences before analysis
deseq2
normalization
9 months ago
mpuli011
• 0
• updated 9 months ago
Michael Love
31k
0
Votes
1
Reply
61
Views
Length scaled TPM and orthologous genes, from Tximport package
normalization
9 months ago
cossardg
• 0
• updated 9 months ago
Michael Love
31k
0
Votes
8
Replies
103
Views
Combining 2 datasets from 2 different RNAseq experiments
deseq2
normalization
probe
9 months ago
l.s.wijaya
• 0
• updated 9 months ago
Michael Love
31k
0
Votes
4
Replies
81
Views
Issues with normalizing viral-infected single cell data
scan
scater
single cell
normalization
9 months ago
Jenny Drnevich
♦ 2.0k
• updated 9 months ago
Aaron Lun
♦ 26k
0
Votes
3
Replies
88
Views
DEseq2 design tutorial with multiple experimental factors
deseq2
annotation
normalization
9 months ago
christophe.vanhaver
• 0
2
Votes
1
Reply
54
Views
Using DESeq2 for normalization of dataset with no replicates
normalization
deseq2
10 months ago
Hadi Hosseini
• 0
• updated 10 months ago
Michael Love
31k
0
Votes
0
Replies
31
Views
DESeq2 analysis with scaled continues variables
normalization
10 months ago
Do it!
• 0
1,636 results • Page
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Comment: Replace a specific nucleotide sequence, at a specific position, with another in
by
Konstantinos Yeles
• 40
Hello @Benjamin-24571 for your example, I can suggest you this code: Read <- c("1","2","3","4") Sequences <- c("ACACCCACG", "AA…
Comment: CHIP Seq Analysis by DiffBind Package
by
shrinka.genetics
• 0
Hello Thanks for your reply. I changed my file format and now it ran. It gave one plot also. All file s are in same directory. No…
Answer: Installing Bioconductor packages in Docker
by
Konstantinos Yeles
• 40
Hello, @adklts-21974 My way to do the installation of packages in a docker is like this: FROM bioconductor/bioconductor_docker…
Comment: HTqPCR: dealing with technical replicates (filterCategory) and inability to anal
by
larissa.stanberry
• 0
I ran into the same issue. It appears that filtering does not carry over featureData with the exception of featureNames, e.g. call featureD…
Comment: Design Matrix for DESeq2 or EdgeR
by
Kevin Blighe
2.3k
Cross-posted on Biostars: https://www.biostars.org/p/484823/
Votes
Answer: Biocthis GHA recently fails for macOS
Answer: Limma help (creating design matrix)
Comment: Normalization for two bulk RNA datasets with different library sizes
How to better design a Volcano plot
Replace a specific nucleotide sequence, at a specific position, with another in Biostrings
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